What are the main steps in ELISA?
1. Add sample and control sample to the wells (Ag- / Ag+), Incubate for 5 min ~ Wash
2. Add primary antibody to well and incubate ~ Wash
3. Add secondary antibody ( If the primary antibodies have bound to the antigen, the secondary antibodies will bind tightly to the primary antibodies ~ Wash
4. Add enzyme substrate and wait 5 min. If antigen was present, the wells turn blue (+), if no antigen, wells remain colorless (-)